High-efficiency Agrobacterium-mediated transformation of Norway spruce (Picea abies) and loblolly pine (Pinus taeda).
Identifieur interne : 000900 ( Main/Exploration ); précédent : 000899; suivant : 000901High-efficiency Agrobacterium-mediated transformation of Norway spruce (Picea abies) and loblolly pine (Pinus taeda).
Auteurs : A R Wenck [États-Unis] ; M. Quinn ; R W Whetten ; G. Pullman ; R. SederoffSource :
- Plant molecular biology [ 0167-4412 ] ; 1999.
Descripteurs français
- KwdFr :
- ADN bactérien (analyse), ADN bactérien (génétique), Acétophénones (pharmacologie), Agrobacterium tumefaciens (croissance et développement), Agrobacterium tumefaciens (génétique), Agrobacterium tumefaciens (pathogénicité), Données de séquences moléculaires (MeSH), Pinus taeda (MeSH), Relation dose-effet des médicaments (MeSH), Séquence nucléotidique (MeSH), Techniques de culture (MeSH), Transformation génétique (effets des médicaments et des substances chimiques), Virulence (génétique).
- MESH :
- analyse : ADN bactérien.
- croissance et développement : Agrobacterium tumefaciens.
- effets des médicaments et des substances chimiques : Transformation génétique.
- génétique : ADN bactérien, Agrobacterium tumefaciens, Virulence.
- pathogénicité : Agrobacterium tumefaciens.
- pharmacologie : Acétophénones.
- Données de séquences moléculaires, Pinus taeda, Relation dose-effet des médicaments, Séquence nucléotidique, Techniques de culture.
English descriptors
- KwdEn :
- Acetophenones (pharmacology), Agrobacterium tumefaciens (genetics), Agrobacterium tumefaciens (growth & development), Agrobacterium tumefaciens (pathogenicity), Base Sequence (MeSH), Culture Techniques (MeSH), DNA, Bacterial (analysis), DNA, Bacterial (genetics), Dose-Response Relationship, Drug (MeSH), Molecular Sequence Data (MeSH), Pinus taeda (MeSH), Transformation, Genetic (drug effects), Virulence (genetics).
- MESH :
- chemical , analysis : DNA, Bacterial.
- chemical , genetics : DNA, Bacterial.
- chemical , pharmacology : Acetophenones.
- drug effects : Transformation, Genetic.
- genetics : Agrobacterium tumefaciens, Virulence.
- growth & development : Agrobacterium tumefaciens.
- pathogenicity : Agrobacterium tumefaciens.
- Base Sequence, Culture Techniques, Dose-Response Relationship, Drug, Molecular Sequence Data, Pinus taeda.
Abstract
Agrobacterium-mediated gene transfer is the method of choice for many plant biotechnology laboratories; however, large-scale use of this organism in conifer transformation has been limited by difficult propagation of explant material, selection efficiencies and low transformation frequency. We have analyzed co-cultivation conditions and different disarmed strains of Agrobacterium to improve transformation. Additional copies of virulence genes were added to three common disarmed strains. These extra virulence genes included either a constitutively active virG or extra copies of virG and virB, both from pTiBo542. In experiments with Norway spruce, we increased transformation efficiencies 1000-fold from initial experiments where little or no transient expression was detected. Over 100 transformed lines expressing the marker gene beta-glucuronidase (GUS) were generated from rapidly dividing embryogenic suspension-cultured cells co-cultivated with Agrobacterium. GUS activity was used to monitor transient expression and to further test lines selected on kanamycin-containing medium. In loblolly pine, transient expression increased 10-fold utilizing modified Agrobacterium strains. Agrobacterium-mediated gene transfer is a useful technique for large-scale generation of transgenic Norway spruce and may prove useful for other conifer species.
DOI: 10.1023/a:1006126609534
PubMed: 10092170
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Quinn</name></author><author><name sortKey="Whetten, R W" sort="Whetten, R W" uniqKey="Whetten R" first="R W" last="Whetten">R W Whetten</name></author><author><name sortKey="Pullman, G" sort="Pullman, G" uniqKey="Pullman G" first="G" last="Pullman">G. Pullman</name></author><author><name sortKey="Sederoff, R" sort="Sederoff, R" uniqKey="Sederoff R" first="R" last="Sederoff">R. 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We have analyzed co-cultivation conditions and different disarmed strains of Agrobacterium to improve transformation. Additional copies of virulence genes were added to three common disarmed strains. These extra virulence genes included either a constitutively active virG or extra copies of virG and virB, both from pTiBo542. In experiments with Norway spruce, we increased transformation efficiencies 1000-fold from initial experiments where little or no transient expression was detected. Over 100 transformed lines expressing the marker gene beta-glucuronidase (GUS) were generated from rapidly dividing embryogenic suspension-cultured cells co-cultivated with Agrobacterium. GUS activity was used to monitor transient expression and to further test lines selected on kanamycin-containing medium. In loblolly pine, transient expression increased 10-fold utilizing modified Agrobacterium strains. Agrobacterium-mediated gene transfer is a useful technique for large-scale generation of transgenic Norway spruce and may prove useful for other conifer species.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">10092170</PMID><DateCompleted><Year>1999</Year><Month>04</Month><Day>13</Day></DateCompleted><DateRevised><Year>2019</Year><Month>08</Month><Day>22</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0167-4412</ISSN><JournalIssue CitedMedium="Print"><Volume>39</Volume><Issue>3</Issue><PubDate><Year>1999</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Plant molecular biology</Title><ISOAbbreviation>Plant Mol Biol</ISOAbbreviation></Journal><ArticleTitle>High-efficiency Agrobacterium-mediated transformation of Norway spruce (Picea abies) and loblolly pine (Pinus taeda).</ArticleTitle><Pagination><MedlinePgn>407-16</MedlinePgn></Pagination><Abstract><AbstractText>Agrobacterium-mediated gene transfer is the method of choice for many plant biotechnology laboratories; however, large-scale use of this organism in conifer transformation has been limited by difficult propagation of explant material, selection efficiencies and low transformation frequency. We have analyzed co-cultivation conditions and different disarmed strains of Agrobacterium to improve transformation. Additional copies of virulence genes were added to three common disarmed strains. These extra virulence genes included either a constitutively active virG or extra copies of virG and virB, both from pTiBo542. In experiments with Norway spruce, we increased transformation efficiencies 1000-fold from initial experiments where little or no transient expression was detected. Over 100 transformed lines expressing the marker gene beta-glucuronidase (GUS) were generated from rapidly dividing embryogenic suspension-cultured cells co-cultivated with Agrobacterium. GUS activity was used to monitor transient expression and to further test lines selected on kanamycin-containing medium. In loblolly pine, transient expression increased 10-fold utilizing modified Agrobacterium strains. 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Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Plant Mol Biol</MedlineTA><NlmUniqueID>9106343</NlmUniqueID><ISSNLinking>0167-4412</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000098">Acetophenones</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004269">DNA, Bacterial</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C036483">T-DNA</NameOfSubstance></Chemical><Chemical><RegistryNumber>866P45Y84S</RegistryNumber><NameOfSubstance 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Pullman</name><name sortKey="Quinn, M" sort="Quinn, M" uniqKey="Quinn M" first="M" last="Quinn">M. Quinn</name><name sortKey="Sederoff, R" sort="Sederoff, R" uniqKey="Sederoff R" first="R" last="Sederoff">R. Sederoff</name><name sortKey="Whetten, R W" sort="Whetten, R W" uniqKey="Whetten R" first="R W" last="Whetten">R W Whetten</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Wenck, A R" sort="Wenck, A R" uniqKey="Wenck A" first="A R" last="Wenck">A R Wenck</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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